Electrophoretic Mobility Shift Assay Advantages

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Gel electrophoresis is a commonly used method to separate a group of nucleic acids or proteins based on molecular weight.

Typically, an agarose gel is used when running nucleic acids.

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Established in the mid 1970's, New England Biolabs, Inc.

(NEB) is the industry leader in the discovery and production of enzymes for molecular biology applications and now offers the largest selection of recombinant and native enzymes for genomic research.Band migration of DNA-protein complexes is normally detected with radio-labeled DNA probes to detect a sequence-specific interaction.These probes typically consist of a linear stretch of binding sequences that you are examining.The kit also includes biotin-labeled and unlabeled control DNA and control nuclear extract.Click the Gel Shift tab below for data and more information; kit manuals can be downloaded under the Documents tab.For complete details, click the EMSA Method tab below.The Gelshift Chemiluminescent EMSA Assay Kit includes all the reagents needed to study protein-DNA binding for your sample system.On the other hand, polyacrylamide gel is often used for the separation of proteins.EMSA takes advantage of the fact that under the condition of an even electric field in gel electrophoresis, large nucleic acid-protein complexes will migrate much slower than a singular protein (Figure 1). Say protein x is a basic unit (monomer) and it is able to associate with other protein x’s to form a larger complex called tetramer.To be even more sure that a specific protein is binding to the DNA, researchers can also add monoclonal antibodies specific for the nucleic acid-binding protein in the complex, resulting in a “gel super-shift”.By the way, if you are still confused about how to run a protein-separation gel, check out the following articles: There are alternative methods to study protein-DNA interactions.

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