Enzyme Activity Lab Report

Enzyme Activity Lab Report-73
How they break down poisonous chemicals into harmless substances.Enzymes are proteins that speed up the rate of reactions that would happen at a slower rate with the absence of these enzymes.

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Place 2 ml of hydrogen peroxide in each of 3 clean test tubes and then add each of the three test substances to the tubes.

As you add each test substance, record the reaction rate (0-5) for each tube.1.

We found out that all 3 of the substances contained catalase and to tell which one had the greater amount of catalase was simple to see which one had more bubbles.

The last part of the lab we experimented with different temperatures on the catalase to see if it would affect the reaction.

Put one test tube of liver and one of H2O2 into each of the following water baths: Ice bath and Warm water bath (not boiling).

You will now test for the presence of catalase in tissues other than liver.Observing a normal catalase reaction, we found out that adding catalase to hydrogen peroxide will lead to the release of oxygen in H2O2 leaving us with water.Adding the catalase into the hydrogen peroxide we noticed that the test tube got warmer which makes the reaction exothermic.The purpose of this lab was to see how catalase from different hosts reacted in different environments.We learned that the catalase found in chicken liver reacted less when cooled, and not at all when heated.The optimal ph for catalase is neutral, because that it what most of the body is comprised of.The optimal temperature of catalse is 98.6 degrees Fahrenheit, because that is the average temperature of the human body.Since enzymes are selective for their substrates and speed up only a few reactions from among many possibilities, the set of enzymes made in a cell determines which metabolic pathways occur in that cell. Enzymes work by lowering the activation energy for a reaction, thus dramatically increasing the rate of the reaction.In this lab, we are dealing with the enzyme that is usually found in the cells of many living tissues.The reaction rate for the cold bath made the reaction rate 2 out of 5 because it cooled it down slowing the reaction rate down.The warm bath’s reaction rate was similar to the cold bath making the reaction rate of 2 out of 5.

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