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MT-III, a snake venom GIIA s PLA2, which shares structural and functional features with mammalian GIIA s PLA2s, activates macrophage defense functions including lipid droplet (LDs) formation, organelle involved in both lipid metabolism and inflammatory processes.Macrophages (MΦs) loaded with LDs, termed foam cells, characterize early blood vessel fatty-streak lesions during atherosclerosis.This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited., but the molecular mechanism underlying its proinflammatory action is not fully understood.
Aliquots of the washes were used for total cell counts in a Neubauer chamber after dilution (, ) in Turk solution (0.2% crystal violet dye in 30% acetic acid).
To verify the predominance of macrophages in samples, differential cell counts were performed on smears stained with Hema3.
Donkey serum was obtained from Jackson Immuno Research Laboratories (West Grove, PA, USA).
Triton-X was obtained from Union Carbide (Houston, TX, USA).
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